Immunity Response of Chickens (Gallus gallus domesticus) in the Early Growth Phase Post Injection Lipopolysaccharide (LPS) E-coli ATCC 25922
DOI:
https://doi.org/10.19184/bioedu.v23i2.53710Keywords:
Body Temperature, LPS E-coli, Native Chicken, blood smearAbstract
Local Indonesian chickens are an important source of animal protein, especially in small-scale farming. However, these farms often face challenges with poor hygiene, which can lead to infections. A common bacterial culprit is E. coli, a Gram-negative bacterium whose outer membrane contains lipopolysaccharide (LPS). This LPS acts as an endotoxin, triggering an immune response in the host that causes inflammation and fever. This study investigated the effect of LPS on the body temperature of native chickens. LPS was extracted using a hot phenol-water method and then injected into the abdominal cavities of chickens at 14, 28, and 32 days of age. Body temperature was measured 12 hours after each injection. The results showed a significant increase in body temperature in LPS-injected chickens compared to the control group. This difference persisted across all three injections, indicating that LPS effectively stimulates the immune system. Additional support for immune stimulation came from the leukocyte differential, which revealed a notable difference in Basophil percentage in the treated chicks. Interestingly, repeated LPS stimulation also led to significant changes in overall leukocyte differentiation. These findings collectively demonstrate that LPS injection successfully enhances the immuno-competence of chicks.
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