The Effect Of Salt Solution Concentration On The Germination And Growth Of Water Lettuce (Lactuca Sativa Var. Angustana)

Authors

  • Siti Safinatun Najeh Universitas PGRI Argopuro Jember
  • Sarwo Danuji Universitas PGRI Argopuro Jember
  • Lila Maharani Universitas PGRI Argopuro Jember

DOI:

https://doi.org/10.19184/bioedu.v23i3.53712

Keywords:

Germination, Growth , Salt solution, Water lettuce (Lactuca sativa var. angustana)

Abstract

Salinity is one of the main obstacles in plant cultivation, especially during the germination and early growth stages. High salt concentrations can cause osmotic stress and ionic toxicity, thereby hindering water absorption and disrupting the physiological processes of the plant. This study aims to examine the effects of various concentrations of salt solution (NaCl) on the germination and early growth processes of water lettuce (Lactuca sativa var. angustana) in a hydroponic system. Soil salinity is a major problem in agriculture that can cause osmotic stress and ionic toxicity in plants, especially at the early growth stages such as germination. This condition hinders water absorption by the seeds and disrupts the balance of essential ions that play a role in cellular activities, thereby reducing the efficiency of physiological processes crucial for the formation of uniform and healthy plants. The research was conducted using a Randomized Complete Block Design. Complete with five treatments of NaCl concentration (0, 25, 50, 75, and 100 mM), each repeated three times. Seeds were sown on cotton media and watered with the solution according to the treatment for 14 days. The observed parameters include germination percentage, plant height, leaf count, and wet weight. The results show that the increase in NaCl concentration is inversely proportional to all growth parameters, where the treatment without NaCl yielded the best results, while the 100 mM concentration provided the lowest values. Statistical analysis confirmed the significant effect of salt concentration on the reduction of seed viability and vegetative growth of watercress. In conclusion, high salinity negatively impacts the physiology and early development of watercress through osmotic stress and ion toxicity mechanisms. These findings are important for the development of plant cultivation techniques in high-salinity areas and support the development of varieties that are more resistant to environmental stress, particularly in intensive agricultural systems such as hydroponics.

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References

Abbas, T., Khan, M. A., Ullah, S., & Rauf, A. (2024). Salt stress and seed germination: Physiological mechanisms and crop management strategies. Journal of Plant Stress Physiology, 14(2), 87–100. https://doi.org/10.1016/j.jpsp.2024.02.005

Banu, M. A., Rahman, M. H., & Alam, M. J. (2023). Salinity stress in agriculture: Impacts and management approaches under climate change scenario. Environmental Sustainability, 6(3), 145–158. https://doi.org/10.1007/s42398-023-00234-6

Basak, R., Islam, M. S., & Ahmed, F. (2023). Growth and nutritional evaluation of stem lettuce (Lactuca sativa var. angustana) under different hydroponic systems. Journal of Horticultural Research, 31(1), 55–63. https://doi.org/10.2478/johr-2023-0005

Bewley, J. D., Bradford, K. J., Hilhorst, H. W. M., & Nonogaki, H. (2022). Seeds: Physiology of development, germination and dormancy (4th ed.). Springer.

FAO. (2023). Global assessment of salt-affected soils and their management. Food and Agriculture Organization of the United Nations. https://www.fao.org/3/cb9073en/cb9073en.pdf

Hasanuzzaman, M., Bhuyan, M. H. M. B., Zulfiqar, F., Raza, A., & Fujita, M. (2021). Reactive oxygen species and antioxidant defense in plants under abiotic stress: Revisiting the crucial roles and key strategies. Journal of Plant Physiology, 256, 153329. https://doi.org/10.1016/j.jplph.2020.153329

Hossain, M. A., Nahar, K., & Fujita, M. (2023). Salinity-induced oxidative stress in plants: Mechanisms and mitigation strategies. In M. Hasanuzzaman (Ed.), Plant stress physiology (pp. 145–170). Springer. https://doi.org/10.1007/978-3-031-13149-1_8

Kleiber, T. (2022). Effects of sodium chloride salinity on ion balance and yield of plants grown in soilless culture. Horticulturae, 8(11), 957. https://doi.org/10.3390/horticulturae8110957

Kumar, R., Verma, R., & Singh, S. (2023). Early seedling responses to salinity: A critical phase for salt tolerance screening in crops. Advances in Agronomy, 174, 73–105. https://doi.org/10.1016/bs.agron.2023.03.002

Lee, J. H., Park, S. Y., & Kim, Y. J. (2023). Impact of salinity on seed germination and seedling growth in selected vegetable species. Horticultural Science & Technology, 41(4), 355–365. https://doi.org/10.7235/HORT.2023.21088

Li, Q., Zhang, Y., & Wang, R. (2023). Salinity effects on cellular expansion and enzymatic activity during early seedling development. Plant Cell Reports, 42(1), 112–125. https://doi.org/10.1007/s00299-023-02933-0

Mohamed, A. A., El-Mageed, T. A., & Shalaby, T. A. (2023). Plant physiological adaptations to salinity stress: New insights into crop improvement. Frontiers in Plant Science, 14, 1165785. https://doi.org/10.3389/fpls.2023.1165785

Munns, R., & Tester, M. (2020). Mechanisms of salinity tolerance. Annual Review of Plant Biology, 71, 403–433. https://doi.org/10.1146/annurev-arplant-050718-100005

Parida, A. K., & Das, A. B. (2020). Salt tolerance and salinity effects on plants: A review. Ecotoxicology and Environmental Safety, 187, 109841. https://doi.org/10.1016/j.ecoenv.2019.109841

Ramli, N. A., Ahmad, R., & Jalil, A. (2024). Sustainable farming in saline-prone areas using hydroponic systems: A case study on leafy vegetables. Agronomy, 14(1), 44. https://doi.org/10.3390/agronomy14010044

Raza, A., Razzaq, A., Mehmood, S. S., et al. (2021). Impact of salinity stress on physiological and biochemical traits of plants: A review. Agronomy, 11(5), 930. https://doi.org/10.3390/agronomy11050930

Shao, Y., Wang, L., & Lin, H. (2023). Global agricultural productivity under salt stress: Challenges and opportunities. Journal of Agricultural Science and Technology, 25(1), 12–25. https://doi.org/10.1016/j.jast.2023.01.003

Yadav, S., Irfan, M., & Khan, M. I. R. (2023). Role of environmental stressors in crop production: Strategies for improvement. Environmental and Experimental Botany, 204, 105107. https://doi.org/10.1016/j.envexpbot.2023.105107

Zhang, L., Yang, M., & Zhou, J. (2024). Responses of Lactuca sativa var. angustana to salinity stress in controlled environments. Journal of Plant Nutrition, 47(2), 367–380. https://doi.org/10.1080/01904167.2024.1986547

Zhao, H., Chen, T., & Liu, Z. (2023). Mechanisms of salt-induced stress in crop plants: A molecular perspective. Plant Stress Biology, 1(2), 45–60. https://doi.org/10.1016/j.plsb.2023.100024

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Published

10/27/2025