Identification of Bioactive Compounds and Antibacterial Activity of Papaya Leaf Extract (Carica Papaya L.) as an Inhibitor of Pseudomonas Aeruginosa
DOI:
https://doi.org/10.19184/bioedu.v23i3.53758Keywords:
Antibacterial activity , Bioactive compounds , Carica papaya L., , Papaya leaf extract , Pseudomonas aeruginosaAbstract
This study explores the potential of papaya leaf extract (Carica papaya L.) as a natural antibacterial agent against Pseudomonas aeruginosa. The extraction process was carried out using 96% ethanol through maceration, followed by fractionation with n-hexane and ethyl acetate. Antibacterial activity was assessed using disc diffusion and liquid dilution methods to determine the inhibition zone and the Minimum Inhibitory Concentration (MIC). Phytochemica creening confirmed the presence of alkaloids, flavonoids, saponins, and tannins in all tested fractions. Among them, the n-hexane fraction demonstrated the strongest antibacterial activity, with an inhibition zone of 15.10 mm at a 10% concentration and a MIC at 6%. Further nalysis using Gas Chromatography-Mass Spectrometry (GC-MS) identified 79 compounds in the n-hexane fraction, with several dominant compounds such as hexadecanoic acid methyl ester, pentadecanoic acid 14-methyl methyl ester, and phytol — all known for their antibacterial properties. These results suggest that the n-hexane fraction of papaya leaf extract holds promising potential for development as a natural antibacterial agent, particularly against Pseudomonas aeruginosa.
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